• Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody (STJ23986) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody (STJ23986).
  • Immunohistochemistry analysis of paraffin-embedded rat testis using TriMethyl-Histone H3-K9 antibody (STJ23986) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using TriMethyl-Histone H3-K9 antibody (STJ23986) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K9 rabbit polyclonal antibody (STJ23986) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using TriMethyl-Histone H3-K9 rabbit polyclonal antibody (STJ23986) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K9 rabbit polyclonal antibody (STJ23986) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using TriMethyl-Histone H3-K9 rabbit polyclonal antibody (STJ23986) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody (STJ23986) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Tri-Methyl-Histone H3-K9 antibody (STJ23986)

SKU:
STJ23986

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tri-Methyl-Histone H3-K9 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic trimethylated peptide around K9 of human histone H3 (NP_003520.1).
Immunogen Sequence: ARKST
Background The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene.

Information sourced from Uniprot.org

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