Anti-TRAIP antibody (399-449 aa) (STJ194364)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human TRAIP at the amino acid range 399-449
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase TRAIP (399-449 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRAIP |
| Gene ID | 10293 |
| Uniprot ID | TRAIP_HUMAN |
| Immunogen | Synthesized peptide derived from the human TRAIP at the amino acid range 399-449 |
| Immunogen Region | 399-449 aa |
| Specificity | This antibody detects endogenous levels of TRAIP at Human/Mouse |
Additional Info
| Function | E3 ubiquitin ligase required to protect genome stability in response to replication stress. Acts as a key regulator of interstrand cross-link repair, which takes place when both strands of duplex DNA are covalently tethered together, thereby blocking replication and transcription. Controls the choice between the two pathways of replication-coupled interstrand-cross-link repair by mediating ubiquitination of MCM7 subunit of the CMG helicase complex. Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3. If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase, enabling the Fanconi anemia DNA repair pathway. Only catalyzes ubiquitination of MCM7 when forks converge. Also involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis: promotes ubiquitination of DPCs, leading to their degradation by the proteasome. Has also been proposed to play a role in promoting translesion synthesis by mediating the assembly of 'Lys-63'-linked poly-ubiquitin chains on the Y-family polymerase POLN in order to facilitate bypass of DNA lesions and preserve genomic integrity. The function in translesion synthesis is however controversial. Acts as a regulator of the spindle assembly checkpoint. Also acts as a negative regulator of innate immune signaling by inhibiting activation of NF-kappa-B mediated by TNF. Negatively regulates TLR3/4- and RIG-I-mediated IRF3 activation and subsequent IFNB1 production and cellular antiviral response by promoting 'Lys-48'-linked polyubiquitination of TNK1 leading to its proteasomal degradation. |
| Protein Name | E3 Ubiquitin-Protein Ligase TraipRing Finger Protein 206Traf-Interacting Protein |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusNucleoplasmNucleolusChromosomeCytoplasmPerinuclear RegionIn The NucleusFound In Close Proximity To PcnaSuggesting Localization At Replication FociLocalizes To Dna Damage Sites In Response To Replication Stress |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Traip antibodyAnti-Ring Finger Protein 206 antibodyAnti-Traf-Interacting Protein antibodyAnti-TRAIP antibodyAnti-RNF206 antibodyAnti-TRIP antibody |
Information sourced from Uniprot.org