• Immunofluorescence analysis of NIH-3T3 cells using TRAF6 Rabbit polyclonal antibody (STJ119260) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using TRAF6 antibody (STJ119260) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TRAF6 antibody (50-150) (STJ119260)

SKU:
STJ119260

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRAF6 (50-150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRAF6
Gene ID: 7189
Uniprot ID: TRAF6_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TRAF6 (NP_004611.1).
Immunogen Sequence: MEEIQGYDVEFDPPLESKYE CPICLMALREAVQTPCGHRF CKACIIKSIRDAGHKCPVDN EILLENQLFPDNFAKREILS LMVKCPNEGCLHKMELRHLE D
Tissue Specificity Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Post Translational Modifications Sumoylated on Lys-124, Lys-142 and Lys-453 with SUMO1. Polyubiquitinated on Lys-124 by TRAF3IP2.after cell stimulation with IL17A. Polyubiquitinated on Lys-124.after cell stimulation with IL1B or TGFB. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form. Deubiquitinated by USP10 in a TANK-dependent manner, leading to the negative regulation of NF-kappaB signaling upon DNA damage. LRRC19 induces 'Lys-63' ubiquitination. Ubiquitinated at Lys-319 by the SCF(FBXL2) complex, leading to its degradation by the proteasome. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1 on both 'Lys-48' and 'Lys-63'-linked ubiquitin chains.leading to NF-kappa-B signaling inhibition.
Function E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as ECSIT, IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. Participates also in the TCR signaling by ubiquitinating LAT.
Protein Name Tnf Receptor-Associated Factor 6
E3 Ubiquitin-Protein Ligase Traf6
Interleukin-1 Signal Transducer
Ring Finger Protein 85
Ring-Type E3 Ubiquitin Transferase Traf6
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-166058
Reactome: R-HSA-168638
Reactome: R-HSA-168927
Reactome: R-HSA-193692
Reactome: R-HSA-202424
Reactome: R-HSA-205043
Reactome: R-HSA-209543
Reactome: R-HSA-209560
Reactome: R-HSA-2871837
Reactome: R-HSA-445989
Reactome: R-HSA-450302
Reactome: R-HSA-450321
Reactome: R-HSA-5607764
Reactome: R-HSA-5689880
Reactome: R-HSA-5689896
Reactome: R-HSA-6811558
Reactome: R-HSA-9014325
Reactome: R-HSA-9020702
Reactome: R-HSA-933541
Reactome: R-HSA-933542
Reactome: R-HSA-937039
Reactome: R-HSA-937041
Reactome: R-HSA-937042
Reactome: R-HSA-937072
Reactome: R-HSA-9645460
Reactome: R-HSA-9692916
Reactome: R-HSA-9705671
Reactome: R-HSA-975110
Reactome: R-HSA-975138
Reactome: R-HSA-975144
Reactome: R-HSA-975155
Reactome: R-HSA-975163
Reactome: R-HSA-9758274
Reactome: R-HSA-975871
Cellular Localisation Cytoplasm
Cell Cortex
Nucleus
Lipid Droplet
Found In The Nuclei Of Some Aggressive B-Cell Lymphoma Cell Lines As Well As In The Nuclei Of Both Resting And Activated T- And B-Lymphocytes
Found In Punctate Nuclear Body Protein Complexes
Ubiquitination May Occur In The Cytoplasm And Sumoylation In The Nucleus
Rsad2/Viperin Recruits It To The Lipid Droplet
Alternative Antibody Names Anti-Tnf Receptor-Associated Factor 6 antibody
Anti-E3 Ubiquitin-Protein Ligase Traf6 antibody
Anti-Interleukin-1 Signal Transducer antibody
Anti-Ring Finger Protein 85 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Traf6 antibody
Anti-TRAF6 antibody
Anti-RNF85 antibody

Information sourced from Uniprot.org

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