| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TNF receptor-associated factor 3 (240-289 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRAF3 |
| Gene ID: |
7187 |
| Uniprot ID: |
TRAF3_HUMAN |
| Immunogen Region: |
240-289 aa |
| Specificity: |
TRAF3 Polyclonal Antibody detects endogenous levels of TRAF3 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human TRAF3 at the amino acid range 240-289 |
| Function | Cytoplasmic E3 ubiquitin ligase that regulates various signaling pathways, such as the NF-kappa-B, mitogen-activated protein kinase (MAPK) and interferon regulatory factor (IRF) pathways, and thus controls a lot of biological processes in both immune and non-immune cell types. In TLR and RLR signaling pathways, acts as an E3 ubiquitin ligase promoting the synthesis of 'Lys-63'-linked polyubiquitin chains on several substrates such as ASC that lead to the activation of the type I interferon response or the inflammasome. Following the activation of certain TLRs such as TLR4, acts as a negative NF-kappa-B regulator, possibly to avoid unregulated inflammatory response, and its degradation via 'Lys-48'-linked polyubiquitination is required for MAPK activation and production of inflammatory cytokines. Alternatively, when TLR4 orchestrates bacterial expulsion, TRAF3 undergoes 'Lys-33'-linked polyubiquitination and subsequently binds to RALGDS, mobilizing the exocyst complex to rapidly expel intracellular bacteria back for clearance. Acts also as a constitutive negative regulator of the alternative NF-kappa-B pathway, which controls B-cell survival and lymphoid organ development. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. |
| Protein Name | Tnf Receptor-Associated Factor 3Cd40 Receptor-Associated Factor 1Craf1Cd40-Binding ProteinCd40bpLmp1-Associated Protein 1Lap1Ring-Type E3 Ubiquitin Transferase Traf3 |
| Database Links | Reactome: R-HSA-5602571Reactome: R-HSA-5668541Reactome: R-HSA-5676594Reactome: R-HSA-5689896Reactome: R-HSA-9013973Reactome: R-HSA-918233Reactome: R-HSA-936440Reactome: R-HSA-936964Reactome: R-HSA-9692916Reactome: R-HSA-9705671 |
| Cellular Localisation | CytoplasmEndosomeMitochondrionUndergoes Endocytosis Together With Tlr4 Upon Lps SignalingCo-Localized To Mitochondria With Trim35 |
| Alternative Antibody Names | Anti-Tnf Receptor-Associated Factor 3 antibodyAnti-Cd40 Receptor-Associated Factor 1 antibodyAnti-Craf1 antibodyAnti-Cd40-Binding Protein antibodyAnti-Cd40bp antibodyAnti-Lmp1-Associated Protein 1 antibodyAnti-Lap1 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Traf3 antibodyAnti-TRAF3 antibodyAnti-CAP-1 antibodyAnti-CRAF1 antibodyAnti-TRAFAMN antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
