| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC/IF/FC |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-TRAF2 (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 50kDa band observed in Human Ovary and Mouse Testis lysates and in lysates of cell lines HepG2 and Jurkat. Preliminary testing also showed a band at approx 50kDa in U937, NIH3T |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | TRAF2 |
| Gene ID: | 7186 |
| Uniprot ID: | TRAF2_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_066961.2 |
| Immunogen Sequence: | KMEAKNSYVRDD |
| Function | Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation.this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE. |
| Protein Name | Tnf Receptor-Associated Factor 2 E3 Ubiquitin-Protein Ligase Traf2 Ring-Type E3 Ubiquitin Transferase Traf2 Tumor Necrosis Factor Type 2 Receptor-Associated Protein 3 |
| Database Links | Reactome: R-HSA-140534 Reactome: R-HSA-3371378 Reactome: R-HSA-5213460 Reactome: R-HSA-5218900 Reactome: R-HSA-5357786 Reactome: R-HSA-5357905 Reactome: R-HSA-5357956 Reactome: R-HSA-5668541 Reactome: R-HSA-5675482 Reactome: R-HSA-5676594 Reactome: R-HSA-5689880 Reactome: R-HSA-69416 Reactome: R-HSA-75893 Reactome: R-HSA-933541 Reactome: R-HSA-933542 Reactome: R-HSA-9693928 Reactome: R-HSA-9758274 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Tnf Receptor-Associated Factor 2 antibody Anti-E3 Ubiquitin-Protein Ligase Traf2 antibody Anti-Ring-Type E3 Ubiquitin Transferase Traf2 antibody Anti-Tumor Necrosis Factor Type 2 Receptor-Associated Protein 3 antibody Anti-TRAF2 antibody Anti-TRAP3 antibody |
Information sourced from Uniprot.org