STJ70554 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum/Golgi and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-TRAC-1/RNF125 antibody (C-Term) (STJ70554)

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STJ70554-100
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-TRAC-1/RNF125 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:8000.
IF: Strong expression of the protein seen in the endoplasmic reticulum/Golgi of HeLa cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of HeLa cells. Recommended conc
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: RNF125
Gene ID: 54941
Uniprot ID: RN125_HUMAN
Immunogen Region: C-Term
Accession Number: NP_060301.2
Immunogen Sequence: RSLLEYVNHSNTT
Post Translational Modifications Autoubiquitinated, leading to its subsequent proteasomal degradation.
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as RIGI, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53. Acts as a negative regulator of type I interferon production by mediating ubiquitination of RIGI at 'Lys-181', leading to RIGI degradation. Mediates ubiquitination and subsequent degradation of p53/TP53. Mediates ubiquitination and subsequent degradation of JAK1. Acts as a positive regulator of T-cell activation.
Protein Name E3 Ubiquitin-Protein Ligase Rnf125
Ring Finger Protein 125
T-Cell Ring Activation Protein 1
Trac-1
Database Links Reactome: R-HSA-936440
Cellular Localisation Golgi Apparatus Membrane
Lipid-Anchor
Shows A Reticular Staining Pattern Within The Cell And Is Probably Expressed At Other Intracellular Membranes In Addition To The Golgi Membrane
Not Detected At The Plasma Membrane
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf125 antibody
Anti-Ring Finger Protein 125 antibody
Anti-T-Cell Ring Activation Protein 1 antibody
Anti-Trac-1 antibody
Anti-RNF125 antibody

Information sourced from Uniprot.org

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