| Short Description : | Rabbit monoclonal Tropomyosin 1 (185-284) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S2MR |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TPM1 |
| Gene ID: | 7168 |
| Uniprot ID: | TPM1_HUMAN |
| Immunogen Region: | 185-284 |
| Immunogen Sequence: | LSEGKCAELEEELKTVTNNL KSLEAQAEKYSQKEDRYEEE IKVLSDKLKEAETRAEFAER SVTKLEKSIDDLEDELYAQK LKYKAISEELDHALNDMTSI |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 185-284 of human Tropomyosin 1 (P09493). |
| Tissue Specificity | Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle. |
| Post Translational Modifications | Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells. |
| Function | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. |
| Protein Name | Tropomyosin Alpha-1 ChainAlpha-TropomyosinTropomyosin-1 |
| Database Links | Reactome: R-HSA-390522Reactome: R-HSA-445355 |
| Cellular Localisation | CytoplasmCytoskeletonAssociates With F-Actin Stress Fibers |
| Alternative Antibody Names | Anti-Tropomyosin Alpha-1 Chain antibodyAnti-Alpha-Tropomyosin antibodyAnti-Tropomyosin-1 antibodyAnti-TPM1 antibodyAnti-C15orf13 antibodyAnti-TMSA antibody |
Information sourced from Uniprot.org
