Anti-TOP2A antibody [IHC112] (STJA0042275)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
General Information
| Short Description | Mouse monoclonal anti-TOP2A for use in IHC-p in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-p |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | IHC112 |
| Conjugation | Unconjugated |
| Formulation | Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide |
| Storage Instruction | Store at 2-8°C for up to 1 year. Do not freeze. |
Target Information
| Gene Symbol | TOP2A |
| Gene ID | 7153 |
| Uniprot ID | TOP2A_HUMAN |
| Specificity | Tested and validated on tonsil, cervix tissue. |
Additional Info
| Tissue Specificity | Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung. Also found in high-grade lymphomas, squamous cell lung tumors and seminomas. |
| Post Translational Modifications | Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. SUMOylated. |
| Function | Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation. |
| Protein Name | Dna Topoisomerase 2-AlphaDna Topoisomerase Ii - Alpha Isozyme |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-4615885 |
| Cellular Localisation | CytoplasmNucleusNucleoplasmNucleolus |
| Alternative Antibody Names | Anti-Dna Topoisomerase 2-Alpha antibodyAnti-Dna Topoisomerase Ii - Alpha Isozyme antibodyAnti-TOP2A antibodyAnti-TOP2 antibody |
Information sourced from Uniprot.org