| Host: | Rabbit |
| Applications: | IF/ICC/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TOP2A |
| Gene ID: | 7153 |
| Uniprot ID: | TOP2A_HUMAN |
| Immunogen Region: | 1314-1503 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1314-1503 of human DNA topoisomerase II alpha (TOP2A) (NP_001058.2). |
| Immunogen Sequence: | ETEPRRAATKTKFTMDLDSD EDFSDFDEKTDDEDFVPSDA SPPKTKTSPKLSNKELKPQK SVVSDLEADDVKGSVPLSSS PPATHFPDETEITNPVPKKN VTVKKTAAKSQSSTSTTGAK KRAAPKGTKRDPALNSGVSQ KPDPAKTKNRRKRKPSTSDD SDSNFEKIVSKAVTSKKSKG ESDDFHMDFD |
| Tissue Specificity | Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung. Also found in high-grade lymphomas, squamous cell lung tumors and seminomas. |
| Post Translational Modifications | Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. SUMOylated. |
| Function | Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation. |
| Protein Name | Dna Topoisomerase 2-Alpha Dna Topoisomerase Ii - Alpha Isozyme |
| Database Links | Reactome: R-HSA-1362277 Reactome: R-HSA-4615885 |
| Cellular Localisation | Cytoplasm Nucleus Nucleoplasm Nucleolus |
| Alternative Antibody Names | Anti-Dna Topoisomerase 2-Alpha antibody Anti-Dna Topoisomerase Ii - Alpha Isozyme antibody Anti-TOP2A antibody Anti-TOP2 antibody |
Information sourced from Uniprot.org