• Western blot analysis of extracts from HeLa cell, using TOMM22 rabbit monoclonal antibody (STJ11102522) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using TOMM22 rabbit monoclonal antibody (STJ11102522) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded human colon using TOMM22 rabbit monoclonal antibody (STJ11102522) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300 Mu g extracts of K-562 cells using 3 Mu g TOMM22 antibody (STJ11102522). Western blot was performed from the immunoprecipitate using TOMM22 antibody (STJ11102522) at a dilution of 1:1000.

Anti-TOMM22 antibody (1-100) [S2MR] (STJ11102522)

SKU:
STJ11102522

£146.50 - £639.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TOMM22 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOMM22
Gene ID: 56993
Uniprot ID: TOM22_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOMM22 (Q9NS69).
Immunogen Sequence: MAAAVAAAGAGEPQSPDELL PKGDAEKPEEELEEDDDEEL DETLSERLWGLTEMFPERVR SAAGATFDLSLFVAQKMYRF SRAALWIGTTSFMILVLPVV
Tissue Specificity Ubiquitous.
Function Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Protein Name Mitochondrial Import Receptor Subunit Tom22 Homolog
Htom22
1c9-2
Translocase Of Outer Membrane 22 Kda Subunit Homolog
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-5205685
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Import Receptor Subunit Tom22 Homolog antibody
Anti-Htom22 antibody
Anti-1c9-2 antibody
Anti-Translocase Of Outer Membrane 22 Kda Subunit Homolog antibody
Anti-TOMM22 antibody
Anti-TOM22 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance