• Immunofluorescence analysis of rat heart using Cardiac troponin T (TNNT2) Rabbit monoclonal antibody (STJ11102195) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse heart using Cardiac troponin T (TNNT2) Rabbit monoclonal antibody (STJ11102195) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using Cardiac troponin T (TNNT2) Rabbit monoclonal antibody (STJ11102195) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse skeletal muscle, using Cardiac troponin T (TNNT2) Rabbit monoclonal antibody (STJ11102195) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TNNT2 antibody (199-298) [S5MR] (STJ11102195)

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STJ11102195

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Troponin T (199-298) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNNT2
Gene ID: 7139
Uniprot ID: TNNT2_HUMAN
Immunogen Region: 199-298
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 199-298 of human Cardiac troponin T (TNNT2) (P45379).
Immunogen Sequence: QKQAQTERKSGKRQTEREKK KKILAERRKVLAIDHLNEDQ LREKAKELWQSIYNLEAEKF DLQEKFKQQKYEINVLRNRI NDNQKVSKTRGKAKVTGRWK
Tissue Specificity Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
Post Translational Modifications Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.
Function Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Protein Name Troponin T - Cardiac Muscle
Tntc
Cardiac Muscle Troponin T
Ctnt
Database Links Reactome: R-HSA-390522
Alternative Antibody Names Anti-Troponin T - Cardiac Muscle antibody
Anti-Tntc antibody
Anti-Cardiac Muscle Troponin T antibody
Anti-Ctnt antibody
Anti-TNNT2 antibody

Information sourced from Uniprot.org

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