• Human placental mesenchymal stromal cells modulate IFN-γ and IL-10 secretion by CD4+T cells via CD73, and alleviate intestinal damage in mice with graft-versus-host disease. INTERNATIONAL IMMUNOPHARMACOLOGY Xiying Luan WB Human, mouse intestine, colon NCM460 cell, Caco-2 cell
  • Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
  • Zhao, Guo-Ping, et al. "Imidacloprid increases intestinal permeability by disrupting tight junctions. " Ecotoxicology and Environmental Safety 222 (2021) : 112476.

Anti-TJP1 antibody (1600-1700 aa) (STJ191514)

SKU:
STJ191514

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Mouse/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tight junction protein ZO-1 (1600-1700 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IF 1:100-300
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TJP1
Gene ID: 7082
Uniprot ID: ZO1_HUMAN
Immunogen Region: 1600-1700 aa
Specificity: ZO1 Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from part of the human protein.the amino acid1600-1700
Post Translational Modifications Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ.
Function TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus.
Protein Name Tight Junction Protein Zo-1
Tight Junction Protein 1
Zona Occludens Protein 1
Zonula Occludens Protein 1
Database Links Reactome: R-HSA-191650
Reactome: R-HSA-2028269
Reactome: R-HSA-351906
Reactome: R-HSA-8935964
Reactome: R-HSA-9705677
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Junction
Tight Junction
Gap Junction
Cell Projection
Podosome
Moves From The Cytoplasm To The Cell Membrane Concurrently With Cell-Cell Contact
At Podosomal Sites
Is Predominantly Localized In The Ring Structure Surrounding The Actin Core
Colocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells
Alternative Antibody Names Anti-Tight Junction Protein Zo-1 antibody
Anti-Tight Junction Protein 1 antibody
Anti-Zona Occludens Protein 1 antibody
Anti-Zonula Occludens Protein 1 antibody
Anti-TJP1 antibody
Anti-ZO1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance