• Immunoprecipitation analysis of 600 Mu g extracts of Rat brain cells using 3 Mu g Timm22 antibody (STJ11103470). Western blot was performed from the immunoprecipitate using Timm22 antibody (STJ11103470) at a dilution of 1:500.
  • Western blot analysis of various lysates using Timm22 Rabbit polyclonal antibody (STJ11103470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Timm22 antibody (19-121) (STJ11103470)

SKU:
STJ11103470

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Timm22
Gene ID: 56322
Uniprot ID: TIM22_MOUSE
Immunogen Region: 19-121
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 19-121 of mouse Timm22 (NP_062792.2).
Immunogen Sequence: AEAPLQYSLLLQYLVGDKRQ PRLLEPGSLGGIPSPAKSEE QKMIERAMESCAFKAVLACV GGFVLGGAFGIFTAGIDTNV GFDPKDPYRTPTAKEVLKDM GQR
Post Translational Modifications Disulfide bonds promote efficient assembly of the TIM22 complex.
Function Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim22
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim22 antibody
Anti-Timm22 antibody
Anti-Tim22 antibody

Information sourced from Uniprot.org

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