| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TIGAR |
| Gene ID: | 57103 |
| Uniprot ID: | TIGAR_HUMAN |
| Immunogen Region: | 100-200 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TIGAR (NP_065108.1). |
| Immunogen Sequence: | LSELRAMAKAAREECPVFTP PGGETLDQVKMRGIDFFEFL CQLILKEADQKEQFSQGSPS NCLETSLAEIFPLGKNHSSK VNSDSGIPGLAASVLVVSHG A |
| Tissue Specificity | Expressed in the brain. Expressed in breast tumors. Expressed in glioblastomas. |
| Function | Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate. Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production. Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death. Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity. In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage. Plays a role in adult intestinal regeneration.contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation. Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions. Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner. Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses. Involved in intestinal tumor progression. |
| Protein Name | Fructose-2 -6-Bisphosphatase Tigar Tp53-Induced Glycolysis And Apoptosis Regulator Tp53-Induced Glycolysis Regulatory Phosphatase |
| Database Links | Reactome: R-HSA-5628897 |
| Cellular Localisation | Cytoplasm Nucleus Mitochondrion Translocated To The Mitochondria During Hypoxia In A Hif1a-Dependent Manner Colocalizes With Hk2 In The Mitochondria During Hypoxia Translocated To The Nucleus During Hypoxia And/Or Genome Stress-Induced Dna Damage Responses In Cancer Cells Translocation To The Mitochondria Is Enhanced In Ischemic Cortex After Reperfusion And/Or During Oxygen And Glucose Deprivation (Ogd)/Reoxygenation Insult In Primary Neurons |
| Alternative Antibody Names | Anti-Fructose-2 -6-Bisphosphatase Tigar antibody Anti-Tp53-Induced Glycolysis And Apoptosis Regulator antibody Anti-Tp53-Induced Glycolysis Regulatory Phosphatase antibody Anti-TIGAR antibody Anti-C12orf5 antibody |
Information sourced from Uniprot.org