• Immunofluorescence analysis of HeLa cells using TIF1 Gamma monoclonal antibody.
  • Western blot analysis using TIF1 Gamma monoclonal antibody against MCF7 cell lysate, HeLa nuclear extract.

Anti-TRIM33 antibody (C-Term) (STJ98564)

SKU:
STJ98564

Current Stock:
Host: Mouse
Applications: WB/IF/IHC
Reactivity: Human/Mouse/Rat/Cow/Dog/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-E3 ubiquitin-protein ligase TRIM33 (C-Term) is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Conjugation: Unconjugated
Formulation: Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol.
Purification: Affinity purification
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-1:2000
IF 1:100-1:500
IHC 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM33
Gene ID: 51592
Uniprot ID: TRI33_HUMAN
Immunogen Region: C-Term
Specificity: TIF1 Gamma Monoclonal Antibody detects endogenous levels of TIF1 Gamma protein.
Immunogen: Purified recombinant human TIF1 Gamma (C-terminus) protein fragments expressed in E.coli.
Post Translational Modifications Sumoylated with SUMO1.
Function Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
Protein Name E3 Ubiquitin-Protein Ligase Trim33
Ectodermin Homolog
Ret-Fused Gene 7 Protein
Protein Rfg7
Ring-Type E3 Ubiquitin Transferase Trim33
Transcription Intermediary Factor 1-Gamma
Tif1-Gamma
Tripartite Motif-Containing Protein 33
Database Links Reactome: R-HSA-2173795
Reactome: R-HSA-9754189
Cellular Localisation Nucleus
In Discrete Nuclear Dots Resembling Nuclear Bodies
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim33 antibody
Anti-Ectodermin Homolog antibody
Anti-Ret-Fused Gene 7 Protein antibody
Anti-Protein Rfg7 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Trim33 antibody
Anti-Transcription Intermediary Factor 1-Gamma antibody
Anti-Tif1-Gamma antibody
Anti-Tripartite Motif-Containing Protein 33 antibody
Anti-TRIM33 antibody
Anti-KIAA1113 antibody
Anti-RFG7 antibody
Anti-TIF1G antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance