| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TIAM1 |
| Gene ID: | 7074 |
| Uniprot ID: | TIAM1_HUMAN |
| Immunogen Region: | 1392-1591 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1392-1591 of human TIAM1 (NP_003244.2). |
| Immunogen Sequence: | SILRDKHRRQLLKTESLPSS QQYVPFGGKRLCALKGARPA MSRAVSAPSKSLGRRRRRLA RNRFTIDSDAVSASSPEKES QQPPGGGDTDRWVEEQFDLA QYEEQDDIKETDILSDDDEF CESVKGASVDRDLQERLQAT SISQRERGRKTLDSHASRMA QLKKQAALSGINGGLESASE EVIWVRREDFAPSRKLNTEI |
| Tissue Specificity | Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas. |
| Post Translational Modifications | Ubiquitinated. Undergoes 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 by a CUL3(KBTBD6/7) E3 ubiquitin ligase complex composed of CUL3, RBX1, KBTBD6 and KBTBD7. 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 triggers proteasomal degradation. Ubiquitination at Lys-1404 and Lys-1420 by CUL3(KBTBD6/7) also requires the membrane-associated protein GABARAP and may therefore be spatially restricted within the cell. |
| Function | Guanyl-nucleotide exchange factor that activates RHO-like proteins and connects extracellular signals to cytoskeletal activities. Activates RAC1, CDC42, and to a lesser extent RHOA and their downstream signaling to regulate processes like cell adhesion and cell migration. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor Tiam1T-Lymphoma Invasion And Metastasis-Inducing Protein 1Tiam-1 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-3928662Reactome: R-HSA-3928665Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423Reactome: R-HSA-9032845 |
| Cellular Localisation | Cell JunctionCell MembranePeripheral Membrane ProteinCytoplasmic SideDetected At The Boundary Between Cells With Actin-Rich ProtrusionsPresence Of Krit1Cdh5 And Rap1b Is Required For Its Localization To The Cell Junction |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor Tiam1 antibodyAnti-T-Lymphoma Invasion And Metastasis-Inducing Protein 1 antibodyAnti-Tiam-1 antibodyAnti-TIAM1 antibody |
Information sourced from Uniprot.org