Host: |
Rabbit |
Applications: |
WB/IHC |
Reactivity: |
Human/Rat |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Rabbit polyclonal antibody anti-TIAM1 (1392-1591) is suitable for use in Western Blot and Immunohistochemistry research applications. |
Clonality: |
Polyclonal |
Conjugation: |
Unconjugated |
Isotype: |
IgG |
Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
Purification: |
Affinity purification |
Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: |
TIAM1 |
Gene ID: |
7074 |
Uniprot ID: |
TIAM1_HUMAN |
Immunogen Region: |
1392-1591 |
Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1392-1591 of human TIAM1 (NP_003244.2). |
Immunogen Sequence: |
SILRDKHRRQLLKTESLPSS QQYVPFGGKRLCALKGARPA MSRAVSAPSKSLGRRRRRLA RNRFTIDSDAVSASSPEKES QQPPGGGDTDRWVEEQFDLA QYEEQDDIKETDILSDDDEF CESVKGASVDRDLQERLQAT SISQRERGRKTLDSHASRMA QLKKQAALSGINGGLESASE EVIWVRREDFAPSRKLNTEI |
Tissue Specificity | Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas. |
Post Translational Modifications | Ubiquitinated. Undergoes 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 by a CUL3(KBTBD6/7) E3 ubiquitin ligase complex composed of CUL3, RBX1, KBTBD6 and KBTBD7. 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 triggers proteasomal degradation. Ubiquitination at Lys-1404 and Lys-1420 by CUL3(KBTBD6/7) also requires the membrane-associated protein GABARAP and may therefore be spatially restricted within the cell. |
Function | Guanyl-nucleotide exchange factor that activates RHO-like proteins and connects extracellular signals to cytoskeletal activities. Activates RAC1, CDC42, and to a lesser extent RHOA and their downstream signaling to regulate processes like cell adhesion and cell migration. |
Protein Name | Rho Guanine Nucleotide Exchange Factor Tiam1T-Lymphoma Invasion And Metastasis-Inducing Protein 1Tiam-1 |
Database Links | Reactome: R-HSA-193648Reactome: R-HSA-3928662Reactome: R-HSA-3928665Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423Reactome: R-HSA-9032845 |
Cellular Localisation | Cell JunctionCell MembranePeripheral Membrane ProteinCytoplasmic SideDetected At The Boundary Between Cells With Actin-Rich ProtrusionsPresence Of Krit1Cdh5 And Rap1b Is Required For Its Localization To The Cell Junction |
Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor Tiam1 antibodyAnti-T-Lymphoma Invasion And Metastasis-Inducing Protein 1 antibodyAnti-Tiam-1 antibodyAnti-TIAM1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance