| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | THEMIS2 |
| Gene ID: | 9473 |
| Uniprot ID: | THMS2_HUMAN |
| Immunogen Region: | 1-260 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human THEMIS2 (NP_004839.2). |
| Immunogen Sequence: | MEPVPLQDFVRALDPASLPR VLRVCSGVYFEGSIYEISGN ECCLSTGDLIKVTQVRLQKV VCENPKTSQTMELAPNFQGY FTPLNTPQSYETLEELVSAT TQSSKQLPTCFMSTHRIVTE GRVVTEDQLLMLEAVVMHLG IRSARCVLGMEGQQVILHLP LSQKGPFWTWEPSAPRTLLQ VLQDPALKDLVLTCPTLPWH SLILRPQYEIQAIMHIFSSL RIAATRSAAQTQGEDLARV |
| Tissue Specificity | Expressed in different endometrial adenocarcinoma cell lines and various other cell lines apart from the prostate cell line LNCaP and the ovarian cancer cell line BG1. |
| Post Translational Modifications | Phosphorylation at Tyr-632 is induced by LPS. Phosphorylated by Src kinases (Lck or Fyn) following BCR engagement. |
| Function | May constitute a control point in macrophage inflammatory response, promoting LPS-induced TLR4-mediated TNF production. Determines the threshold for activation of B cells by low-affinity and low-avidity ligands via PLCG2 activation and its downstream pathways. |
| Protein Name | Protein Themis2Induced By Contact To Basement Membrane 1 ProteinProtein Icb-1Thymocyte-Expressed Molecule Involved In Selection Protein 2 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Protein Themis2 antibodyAnti-Induced By Contact To Basement Membrane 1 Protein antibodyAnti-Protein Icb-1 antibodyAnti-Thymocyte-Expressed Molecule Involved In Selection Protein 2 antibodyAnti-THEMIS2 antibodyAnti-C1orf38 antibodyAnti-ICB1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of various lysates using [KO Validated] WSTF Rabbit pAb (STJ111893) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/91989/409014/STJ111893_1__41746.1744895409.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] WSTF Rabbit pAb (STJ111893) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.")
