| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:5000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TH |
| Gene ID: | 7054 |
| Uniprot ID: | TY3H_HUMAN |
| Immunogen Region: | 411-528 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 411-528 of human Tyrosine Hydroxylase (NP_954986.2). |
| Immunogen Sequence: | KQNGEVKAYGAGLLSSYGEL LHCLSEEPEIRAFDPEAAAV QPYQDQTYQSVYFVSESFSD AKDKLRSYASRIQRPFSVKF DPYTLAIDVLDSPQAVRRSL EGVQDELDTLAHALSAIG |
| Tissue Specificity | Mainly expressed in the brain and adrenal glands. |
| Post Translational Modifications | Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities. Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity. Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines. Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway. Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network. Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG.this interaction may influence the phosphorylation and dephosphorylation of other sites. Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity. |
| Function | Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity. Positively regulates the regression of retinal hyaloid vessels during postnatal development. Isoform 5: Lacks catalytic activity. Isoform 6: Lacks catalytic activity. |
| Protein Name | Tyrosine 3-Monooxygenase Tyrosine 3-Hydroxylase Th |
| Database Links | Reactome: R-HSA-209905 |
| Cellular Localisation | Cytoplasm Perinuclear Region Nucleus Cell Projection Axon Cytoplasmic Vesicle Secretory Vesicle Synaptic Vesicle When Phosphorylated At Ser-19 Shows A Nuclear Distribution And When Phosphorylated At Ser-31 As Well At Ser-40 Shows A Cytosolic Distribution Expressed In Dopaminergic Axons And Axon Terminals |
| Alternative Antibody Names | Anti-Tyrosine 3-Monooxygenase antibody Anti-Tyrosine 3-Hydroxylase antibody Anti-Th antibody Anti-TH antibody Anti-TYH antibody |
Information sourced from Uniprot.org