• Immunofluorescence analysis of U-2 OS cells using TGN46/TGN46/TGOLN2 Rabbit polyclonal antibody (STJ119119) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TGN46/TGOLN2 in paraffin-embedded Human colon using TGN46/TGN46/TGOLN2 Rabbit polyclonal antibody (STJ119119) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TGN46/TGOLN2 in paraffin-embedded Mouse testis using TGN46/TGN46/TGOLN2 Rabbit polyclonal antibody (STJ119119) at dilution of 1:100 (20x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TGN46/TGOLN2 in paraffin-embedded Mouse testis using TGN46/TGN46/TGOLN2 Rabbit polyclonal antibody (STJ119119) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TGN46/TGOLN2 Rabbit polyclonal antibody (STJ119119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-TGOLN2 antibody (323-423) (STJ119119)

SKU:
STJ119119

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TGOLN2 (323-423) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TGOLN2
Gene ID: 10618
Uniprot ID: TGON2_HUMAN
Immunogen Region: 323-423
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 323-423 of human TGN46/TGN46/TGOLN2 (NP_006455.2).
Immunogen Sequence: PKEAEDDDTGPEEGSPPKEE KEKMSGSASSENREGTLSDS TGSEKDDLYPNGSGNGSAES SHFFAYLVTAAILVAVLYIA HHNKRKIIAFVLEGKRSKVT R
Tissue Specificity Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
Function May be involved in regulating membrane traffic to and from trans-Golgi network.
Protein Name Trans-Golgi Network Integral Membrane Protein 2
Trans-Golgi Network Glycoprotein 46
Tgn38 Homolog
Htgn46
Trans-Golgi Network Glycoprotein 48
Htgn48
Trans-Golgi Network Glycoprotein 51
Htgn51
Trans-Golgi Network Protein 2
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-432722
Reactome: R-HSA-6811440
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8957275
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus
Trans-Golgi Network Membrane
Primarily In Trans-Golgi Network
Cycles Between The Trans-Golgi Network And The Cell Surface Returning Via Endosomes
Alternative Antibody Names Anti-Trans-Golgi Network Integral Membrane Protein 2 antibody
Anti-Trans-Golgi Network Glycoprotein 46 antibody
Anti-Tgn38 Homolog antibody
Anti-Htgn46 antibody
Anti-Trans-Golgi Network Glycoprotein 48 antibody
Anti-Htgn48 antibody
Anti-Trans-Golgi Network Glycoprotein 51 antibody
Anti-Htgn51 antibody
Anti-Trans-Golgi Network Protein 2 antibody
Anti-TGOLN2 antibody
Anti-TGN46 antibody
Anti-TGN51 antibody

Information sourced from Uniprot.org

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