• Western blot analysis of extracts of various cell lines, using TFR2 antibody (STJ111887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of HepG2 cells using TFR2 Rabbit polyclonal antibody (STJ111887) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human liver cancer using TFR2 antibody (STJ111887) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using TFR2 antibody (STJ111887) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HepG2 cells using TFR2 rabbit polyclonal antibody (STJ111887) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using TFR2 antibody (STJ111887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TFR2 antibody (130-330) (STJ111887)

SKU:
STJ111887

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TFR2
Gene ID: 7036
Uniprot ID: TFR2_HUMAN
Immunogen Region: 130-330
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 130-330 of human TFR2 (NP_003218.2).
Immunogen Sequence: FHQGRLYWSDLQAMFLQFLG EGRLEDTIRQTSLRERVAGS AGMAALTQDIRAALSRQKLD HVWTDTHYVGLQFPDPAHPN TLHWVDEAGKVGEQLPLEDP DVYCPYSAIGNVTGELVYAH YGRPEDLQDLRARGVDPVGR LLLVRVGVISFAQKVTNAQD FGAQGVLIYPEPADFSQDPP KPSLSSQQAVYGHVHLGTGD P
Tissue Specificity Predominantly expressed in liver. While the alpha form is also expressed in spleen, lung, muscle, prostate and peripheral blood mononuclear cells, the beta form is expressed in all tissues tested, albeit weakly.
Function Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation.
Protein Name Transferrin Receptor Protein 2
Tfr2
Database Links Reactome: R-HSA-917977
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Isoform Beta: Cytoplasm
Lacks The Transmembrane Domain
Probably Intracellular
Alternative Antibody Names Anti-Transferrin Receptor Protein 2 antibody
Anti-Tfr2 antibody
Anti-TFR2 antibody

Information sourced from Uniprot.org

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