Anti-TERF1 antibody [R05-4G3] (STJA0036304)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human TRF1
STJA0036304
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General Information

Short DescriptionRabbit monoclonal anti-TERF1 for use in WB, ICC and IF in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ICC/IF
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR05-4G3
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity Chromatography
Dilution RangeWB 1:500-1:1000
IF 1:50-1:200
FormulationRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolTERF1
Gene ID7013
Uniprot IDTERF1_HUMAN
ImmunogenA synthesized peptide derived from human TRF1

Additional Info

Tissue Specificity Highly expressed and ubiquitous. Isoform Pin2 predominates.
Post Translational Modifications Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage. ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA. Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Function Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends.without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Protein Name Telomeric Repeat-Binding Factor 1
Nima-Interacting Protein 2
Ttaggg Repeat-Binding Factor 1
Telomeric Protein Pin2/Trf1
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-1221632
Reactome: R-HSA-171306
Reactome: R-HSA-171319
Reactome: R-HSA-174411
Reactome: R-HSA-174414
Reactome: R-HSA-174417
Reactome: R-HSA-174430
Reactome: R-HSA-174437
Reactome: R-HSA-2559586
Reactome: R-HSA-9670095
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Spindle
Chromosome
Telomere
Colocalizes With Telomeric Dna In Interphase And Prophase Cells
Telomeric Localization Decreases In Metaphase
Anaphase And Telophase
Associates With The Mitotic Spindle
Colocalizes With Triobp Isoform 1 At The Telomeres In Interphase
Alternative Antibody Names Anti-Telomeric Repeat-Binding Factor 1 antibody
Anti-Nima-Interacting Protein 2 antibody
Anti-Ttaggg Repeat-Binding Factor 1 antibody
Anti-Telomeric Protein Pin2/Trf1 antibody
Anti-TERF1 antibody
Anti-PIN2 antibody
Anti-TRBF1 antibody
Anti-TRF antibody
Anti-TRF1 antibody

Information sourced from Uniprot.org

Citations

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