• Confocal immunofluorescence analysis of U-2 OS cells using TEAD3 Rabbit polyclonal antibody (STJ29590) at dilution of 1:400. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A549 cells using TEAD3 Rabbit polyclonal antibody (STJ29590). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.
  • Immunohistochemistry analysis of TEAD3 in paraffin-embedded human placenta using TEAD3 Rabbit polyclonal antibody (STJ29590) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TEAD3 Rabbit polyclonal antibody (STJ29590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TEAD3 antibody (130-330) (STJ29590)

SKU:
STJ29590

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TEAD3 (130-330) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TEAD3
Gene ID: 7005
Uniprot ID: TEAD3_HUMAN
Immunogen Region: 130-330
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-330 of human TEAD3 (NP_003205.2).
Immunogen Sequence: SSAQIVSASVLQNKFSPPSP LPQAVFSTSSRFWSSPPLLG QQPGPSQDIKPFAQPAYPIQ PPLPPTLSSYEPLAPLPSAA ASVPVWQDRTIASSRLRLLE YSAFMEVQRDPDTYSKHLFV HIGQTNPAFSDPPLEAVDVR QIYDKFPEKKGGLKELYEKG PPNAFFLVKFWADLNSTIQE GPGAFYGVSSQYSSADSMTI S
Tissue Specificity Preferentially expressed in the placenta.
Function Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to multiple functional elements of the human chorionic somatomammotropin-B gene enhancer.
Protein Name Transcriptional Enhancer Factor Tef-5
Dtef-1
Tea Domain Family Member 3
Tead-3
Database Links Reactome: R-HSA-2032785
Reactome: R-HSA-8951671
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcriptional Enhancer Factor Tef-5 antibody
Anti-Dtef-1 antibody
Anti-Tea Domain Family Member 3 antibody
Anti-Tead-3 antibody
Anti-TEAD3 antibody
Anti-TEAD5 antibody
Anti-TEF5 antibody

Information sourced from Uniprot.org

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