• Western blot analysis of Jurkat, using TCF12 antibody (STJ25790) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of mouse thymus, using TCF12 antibody (STJ25790) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TCF12 antibody (443-682) (STJ25790)

SKU:
STJ25790

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TCF12 (443-682) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TCF12
Gene ID: 6938
Uniprot ID: HTF4_HUMAN
Immunogen Region: 443-682
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 443-682 of human TCF12 (NP_003196.1).
Immunogen Sequence: PIGSLNSNYGGSSLVASSRS ASMVGTHREDSVSLNGNHSV LSSTVTTSSTDLNHKTQENY RGGLQSQSGTVVTTEIKTEN KEKDENLHEPPSSDDMKSDD ESSQKDIKVSSRGRTSSTNE DEDLNPEQKIEREKERRMAN NARERLRVRDINEAFKELGR MCQLHLKSEKPQTKLLILHQ AVAVILSLEQQVRERNLNPK AACLKRREEEKVSAVSAEPP TTLPGTHPGLSETTNPMGH
Tissue Specificity Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line.
Function Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). May be involved in the functional network that regulates the development of the GnRH axis.
Protein Name Transcription Factor 12
Tcf-12
Class B Basic Helix-Loop-Helix Protein 20
Bhlhb20
Dna-Binding Protein Htf4
E-Box-Binding Protein
Transcription Factor Htf-4
Database Links Reactome: R-HSA-525793
Reactome: R-HSA-8939236
Reactome: R-HSA-9031628
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor 12 antibody
Anti-Tcf-12 antibody
Anti-Class B Basic Helix-Loop-Helix Protein 20 antibody
Anti-Bhlhb20 antibody
Anti-Dna-Binding Protein Htf4 antibody
Anti-E-Box-Binding Protein antibody
Anti-Transcription Factor Htf-4 antibody
Anti-TCF12 antibody
Anti-BHLHB20 antibody
Anti-HEB antibody
Anti-HTF4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance