• Confocal imaging of paraffin-embedded Mouse brain using TBR1 Rabbit monoclonal antibody (STJ11103036, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using TBR1 Rabbit monoclonal antibody (STJ11103036) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TBR1 antibody (1-100) [S6MR] (STJ11103036)

SKU:
STJ11103036

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TBR1 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TBR1
Gene ID: 10716
Uniprot ID: TBR1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TBR1 (Q16650).
Immunogen Sequence: MQLEHCLSPSIMLSKKFLNV SSSYPHSGGSELVLHDHPII STTDNLERSSPLKKITRGMT NQSDTDNFPDSKDSPGDVQR SKLSPVLDGVSELRHSFDGS
Tissue Specificity Brain.
Function Transcriptional repressor involved in multiple aspects of cortical development, including neuronal migration, laminar and areal identity, and axonal projection. As transcriptional repressor of FEZF2, it blocks the formation of the corticospinal (CS) tract from layer 6 projection neurons, thereby restricting the origin of CS axons specifically to layer 5 neurons.
Protein Name T-Box Brain Protein 1
T-Brain-1
Tbr-1
Tes-56
Cellular Localisation Nucleus
Alternative Antibody Names Anti-T-Box Brain Protein 1 antibody
Anti-T-Brain-1 antibody
Anti-Tbr-1 antibody
Anti-Tes-56 antibody
Anti-TBR1 antibody

Information sourced from Uniprot.org

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