| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TARBP2 (1-366) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TARBP2 |
| Gene ID: |
6895 |
| Uniprot ID: |
TRBP2_HUMAN |
| Immunogen Region: |
1-366 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human TARBP2 (NP_599150.1). |
| Immunogen Sequence: |
MSEEEQGSGTTTGCGLPSIE QMLAANPGKTPISLLQEYGT RIGKTPVYDLLKAEGQAHQP NFTFRVTVGDTSCTGQGPSK KAAKHKAAEVALKHLKGGSM LEPALEDSSSFSPLDSSLPE DIPVFTAAAAATPVPSVVLT RSPPMELQPPVSPQQSECNP VGALQELVVQKGWRLPEYTV TQESGPAHRKEFTMTCRVER FIEIGSGTSKKLAKRNAAAK MLLRVHTVPLDARDGNEVE |
| Function | Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds in vitro to the PRM1 3'-UTR. Seems to act as a repressor of translation. For some pre-miRNA substrates, may also alter the choice of cleavage site by DICER1. Negatively regulates IRF7-mediated IFN-beta signaling triggered by viral infection by inhibiting the phosphorylation of IRF7 and promoting its 'Lys'-48-linked ubiquitination and degradation. (Microbial infection) Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR. Mediates recruitment of FTSJ3 methyltransferase to HIV-1 RNA, leading to 2'-O-methylation of the viral genome, allowing HIV-1 to escape the innate immune system. |
| Protein Name | Risc-Loading Complex Subunit Tarbp2Tar Rna-Binding Protein 2Trans-Activation-Responsive Rna-Binding Protein |
| Database Links | Reactome: R-HSA-203927Reactome: R-HSA-426486 |
| Cellular Localisation | CytoplasmPerinuclear RegionNucleus |
| Alternative Antibody Names | Anti-Risc-Loading Complex Subunit Tarbp2 antibodyAnti-Tar Rna-Binding Protein 2 antibodyAnti-Trans-Activation-Responsive Rna-Binding Protein antibodyAnti-TARBP2 antibodyAnti-TRBP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of extracts of 293T cells, using [KO Validated] SETD7 antibody (STJ112026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/92100/372404/STJ112026_1__54377.1713138378.jpg?c=1 "Western blot analysis of extracts of 293T cells, using [KO Validated] SETD7 antibody (STJ112026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")