• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g TAF6L antibody (STJ116581). Western blot was performed from the immunoprecipitate using TAF6L antibody (STJ116581) at a dilution of 1:1000.
  • Western blot analysis of various lysates using TAF6L Rabbit polyclonal antibody (STJ116581) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TAF6L antibody (1-200) (STJ116581)

SKU:
STJ116581

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TAF6L
Gene ID: 10629
Uniprot ID: TAF6L_HUMAN
Immunogen Region: 1-200
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TAF6L (NP_006464.1).
Immunogen Sequence: MSEREERRFVEIPRESVRLM AESTGLELSDEVAALLAEDV CYRLREATQNSSQFMKHTKR RKLTVEDFNRALRWSSVEAV CGYGSQEALPMRPAREGELY FPEDREVNLVELALATNIPK GCAETAVRVHVSYLDGKGNL APQGSVPSAVSSLTDDLLKY YHQVTRAVLGDDPQLMKVAL QDLQTNSKIGALLPYFVYVV
Function Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex (Probable). With TAF5L, acts as an epigenetic regulator essential for somatic reprogramming. Regulates target genes through H3K9ac deposition and MYC recruitment which trigger MYC regulatory network to orchestrate gene expression programs to control embryonic stem cell state. Functions with MYC to activate target gene expression through RNA polymerase II pause release.
Protein Name Taf6-Like Rna Polymerase Ii P300/Cbp-Associated Factor-Associated Factor 65 Kda Subunit 6l
Taf6l
Pcaf-Associated Factor 65-Alpha
Paf65-Alpha
Database Links Reactome: R-HSA-3214847
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Taf6-Like Rna Polymerase Ii P300/Cbp-Associated Factor-Associated Factor 65 Kda Subunit 6l antibody
Anti-Taf6l antibody
Anti-Pcaf-Associated Factor 65-Alpha antibody
Anti-Paf65-Alpha antibody
Anti-TAF6L antibody
Anti-PAF65A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance