• Western blot analysis of extracts of NCI-H460 cells, using Brachyury rabbit monoclonal antibody (STJ11102243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using Brachyury rabbit monoclonal antibody (STJ11102243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunofluorescence analysis of NCI-H460 using Brachyury rabbit monoclonal antibody (STJ11102243) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-T antibody (266-435) [S3MR] (STJ11102243)

SKU:
STJ11102243

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Brachyury (266-435) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TBXT
Gene ID: 6862
Uniprot ID: TBXT_HUMAN
Immunogen Region: 266-435
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 266-435 of human Brachyury (O15178).
Immunogen Sequence: GALSLPSTHSCDRYPTLRSH RSSPYPSPYAHRNNSPTYSD NSPACLSMLQSHDNWSSLGM PAHPSMLPVSHNASPPTSSS QYPSLWSVSNGAVTPGSQAA AVSNGLGAQFFRGSPAHYTP LTHPVSAPSSSGSPLYEGAA AATDIVDSQYDAAAQGRLIA SWTPVSPPSM
Tissue Specificity Detected in testis, but not in other, normal tissues. Detected in lung tumors (at protein level).
Function Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site.
Protein Name T-Box Transcription Factor T
Brachyury Protein
Protein T
Database Links Reactome: R-HSA-9733709
Reactome: R-HSA-9754189
Reactome: R-HSA-9758919
Reactome: R-HSA-9793380
Reactome: R-HSA-9796292
Reactome: R-HSA-9823730
Cellular Localisation Nucleus
Alternative Antibody Names Anti-T-Box Transcription Factor T antibody
Anti-Brachyury Protein antibody
Anti-Protein T antibody
Anti-TBXT antibody
Anti-T antibody

Information sourced from Uniprot.org

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