• Immunofluorescence analysis of NCI-H460 cells using Brachyury Rabbit polyclonal antibody (STJ119216) aBrachyury diluBrachyuryion of 1:200 (40x lens). Blue: DAPI for nuclear sBrachyuryaining.
  • Western blot analysis of extracts from H460 cells, using Brachyury Rabbit polyclonal antibody (STJ119216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-T antibody (226-435) (STJ119216)

SKU:
STJ119216

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-T (226-435) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TBXT
Gene ID: 6862
Uniprot ID: TBXT_HUMAN
Immunogen Region: 226-435
Immunogen: RecombinanBrachyury fusion proBrachyuryein conBrachyuryaining a sequence corresponding Brachyuryo amino acids 226-435 of human Brachyury (NP_003172.1).
Immunogen Sequence: HKEMMEEPGDSQQPGYSQWG WLLPGTSTLCPPANPHPQFG GALSLPSTHSCDRYPTLRSH RSSPYPSPYAHRNNSPTYSD NSPACLSMLQSHDNWSSLGM PAHPSMLPVSHNASPPTSSS QYPSLWSVSNGAVTPGSQAA AVSNGLGAQFFRGSPAHYTP LTHPVSAPSSSGSPLYEGAA AATDIVDSQYDAAAQGRLIA SWTPVSPPSM
Tissue Specificity Detected in testis, but not in other, normal tissues. Detected in lung tumors (at protein level).
Function Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site.
Protein Name T-Box Transcription Factor T
Brachyury Protein
Protein T
Database Links Reactome: R-HSA-9733709
Reactome: R-HSA-9754189
Reactome: R-HSA-9758919
Reactome: R-HSA-9793380
Reactome: R-HSA-9796292
Reactome: R-HSA-9823730
Cellular Localisation Nucleus
Alternative Antibody Names Anti-T-Box Transcription Factor T antibody
Anti-Brachyury Protein antibody
Anti-Protein T antibody
Anti-TBXT antibody
Anti-T antibody

Information sourced from Uniprot.org

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