• Chromatin immunoprecipitation analysis of extracts of 293T cell line, using SUV39H2 antibody (STJ28418) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Western blot analysis of various lysates using SUV39H2 Rabbit polyclonal antibody (STJ28418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SUV39H2 antibody (141-350) (STJ28418)

SKU:
STJ28418

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUV39H2 (141-350) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUV39H2
Gene ID: 79723
Uniprot ID: SUV92_HUMAN
Immunogen Region: 141-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 141-350 of human SUV39H2 (NP_001180354.1).
Immunogen Sequence: CCPAEAGVLLAYNKNQQIKI PPGTPIYECNSRCQCGPDCP NRIVQKGTQYSLCIFRTSNG RGWGVKTLVKIKRMSFVMEY VGEVITSEEAERRGQFYDNK GITYLFDLDYESDEFTVDAA RYGNVSHFVNHSCDPNLQVF NVFIDNLDTRLPRIALFSTR TINAGEELTFDYQMKGSGDI SSDSIDHSPAKKRVRTVCKC GAVTCRGYLN
Post Translational Modifications Ubiquitinated by the DCX(DCAF13) E3 ubiquitin ligase complex, leading to its degradation.
Function Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.
Protein Name Histone-Lysine N-Methyltransferase Suv39h2
Histone H3-K9 Methyltransferase 2
H3-K9-Hmtase 2
Lysine N-Methyltransferase 1b
Suppressor Of Variegation 3-9 Homolog 2
Su(Var3-9 Homolog 2
Database Links Reactome: R-HSA-3214841
Cellular Localisation Nucleus
Chromosome
Centromere
Associates With Centromeric Constitutive Heterochromatin
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Suv39h2 antibody
Anti-Histone H3-K9 Methyltransferase 2 antibody
Anti-H3-K9-Hmtase 2 antibody
Anti-Lysine N-Methyltransferase 1b antibody
Anti-Suppressor Of Variegation 3-9 Homolog 2 antibody
Anti-Su(Var3-9 Homolog 2 antibody
Anti-SUV39H2 antibody
Anti-KMT1B antibody

Information sourced from Uniprot.org

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