• Western blot analysis of extracts of various cell lines, using SUPV3L1 antibody (STJ111992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH-3T3 cells using SUPV3L1 Polyclonal Antibody (STJ111992) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using SUPV3L1 Polyclonal Antibody (STJ111992) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using SUPV3L1 antibody (STJ111992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SUPV3L1 antibody (577-786) (STJ111992)

SKU:
STJ111992

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUPV3L1 (577-786) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUPV3L1
Gene ID: 6832
Uniprot ID: SUV3_HUMAN
Immunogen Region: 577-786
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 577-786 of human SUPV3L1 (NP_003162.2).
Immunogen Sequence: APINKKQPFVCSSLLQFARQ YSRNEPLTFAWLRRYIKWPL LPPKNIKDLMDLEAVHDVLD LYLWLSYRFMDMFPDASLIR DLQKELDGIIQDGVHNITKL IKMSETHKLLNLEGFPSGSQ SRLSGTLKSQARRTRGTKAL GSKATEPPSPDAGELSLASR LVQQGLLTPDMLKQLEKEWM TQQTEHNKEKTESGTHPKGT RRKKKEPDSD
Tissue Specificity Broadly expressed.
Function Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria.regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA.
Protein Name Atp-Dependent Rna Helicase Supv3l1 - Mitochondrial
Suppressor Of Var1 3-Like Protein 1
Suv3-Like Protein 1
Cellular Localisation Nucleus
Mitochondrion Matrix
Mitochondrion Nucleoid
Alternative Antibody Names Anti-Atp-Dependent Rna Helicase Supv3l1 - Mitochondrial antibody
Anti-Suppressor Of Var1 3-Like Protein 1 antibody
Anti-Suv3-Like Protein 1 antibody
Anti-SUPV3L1 antibody
Anti-SUV3 antibody

Information sourced from Uniprot.org

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