Anti-SUPT6H antibody (938-988 aa) (STJ194271)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human SPT6H at the amino acid range 938-988
General Information
| Short Description | Rabbit polyclonal anti-Transcription elongation factor SPT6 (938-988 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SUPT6H |
| Gene ID | 6830 |
| Uniprot ID | SPT6H_HUMAN |
| Immunogen | Synthesized peptide derived from the human SPT6H at the amino acid range 938-988 |
| Immunogen Region | 938-988 aa |
| Specificity | This antibody detects endogenous levels of SPT6H at Human/Mouse |
Additional Info
| Post Translational Modifications | Dephosphorylated at Ser-1530 by the PNUTS-PP1 complex during RNA polymerase II transcription pause-release. |
| Function | Histone H3-H4 chaperone that plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A. |
| Protein Name | Transcription Elongation Factor Spt6Hspt6Histone Chaperone Suppressor Of Ty6Tat-Cotransactivator 2 ProteinTat-Ct2 Protein |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-674695Reactome: R-HSA-75955 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Elongation Factor Spt6 antibodyAnti-Hspt6 antibodyAnti-Histone Chaperone Suppressor Of Ty6 antibodyAnti-Tat-Cotransactivator 2 Protein antibodyAnti-Tat-Ct2 Protein antibodyAnti-SUPT6H antibodyAnti-KIAA0162 antibodyAnti-SPT6H antibody |
Information sourced from Uniprot.org