• Western blot analysis of extracts of HepG2 cells, using SULT2A1 antibody (STJ11103622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of rat liver using SULT2A1 Rabbit monoclonal antibody (STJ11103622) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat liver using SULT2A1 rabbit monoclonal antibody (STJ11103622) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Hep G2 cells, using SULT2A1 antibody (STJ11103622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SULT2A1 antibody (180-285) [S2MR] (STJ11103622)

SKU:
STJ11103622

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SULT2A1 (180-285) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SULT2A1
Gene ID: 6822
Uniprot ID: ST2A1_HUMAN
Immunogen Region: 180-285
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-285 of human SULT2A1 (Q06520).
Immunogen Sequence: LLLSYEELKQDTGRTIEKIC QFLGKTLEPEELNLILKNSS FQSMKENKMSNYSLLSVDYV VDKAQLLRKGVSGDWKNHFT VAQAEDFDKLFQEKMADLPR ELFPWE
Tissue Specificity Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the adrenal than the liver and the kidney.
Post Translational Modifications The N-terminus is blocked.
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Mediates the sulfation of a wide range of steroids and sterols, including pregnenolone, androsterone, DHEA, bile acids, cholesterol and as well many xenobiotics that contain alcohol and phenol functional groups. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Plays an important role in maintening steroid and lipid homeostasis. Plays a key role in bile acid metabolism. In addition, catalyzes the metabolic activation of potent carcinogenic polycyclic arylmethanols.
Protein Name Sulfotransferase 2a1
St2a1
Bile Salt Sulfotransferase
Dehydroepiandrosterone Sulfotransferase
Dhea-St
Dhea-St8
Hydroxysteroid Sulfotransferase
Hst
St2
Sult2a3
Database Links Reactome: R-HSA-156584
Reactome: R-HSA-1989781
Reactome: R-HSA-9753281
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Sulfotransferase 2a1 antibody
Anti-St2a1 antibody
Anti-Bile Salt Sulfotransferase antibody
Anti-Dehydroepiandrosterone Sulfotransferase antibody
Anti-Dhea-St antibody
Anti-Dhea-St8 antibody
Anti-Hydroxysteroid Sulfotransferase antibody
Anti-Hst antibody
Anti-St2 antibody
Anti-Sult2a3 antibody
Anti-SULT2A1 antibody
Anti-HST antibody
Anti-STD antibody

Information sourced from Uniprot.org

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