• Immunofluorescence analysis of L929 cells using SUCLG1 Rabbit polyclonal antibody (STJ117540) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SUCLG1 Rabbit polyclonal antibody (STJ117540) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SUCLG1 Rabbit polyclonal antibody (STJ117540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SUCLG1 antibody (41-346) (STJ117540)

SKU:
STJ117540

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUCLG1
Gene ID: 8802
Uniprot ID: SUCA_HUMAN
Immunogen Region: 41-346
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 41-346 of human SUCLG1 (NP_003840.2).
Immunogen Sequence: CSYTASRQHLYVDKNTKIIC QGFTGKQGTFHSQQALEYGT KLVGGTTPGKGGQTHLGLPV FNTVKEAKEQTGATASVIYV PPPFAAAAINEAIEAEIPLV VCITEGIPQQDMVRVKHKLL RQEKTRLIGPNCPGVINPGE CKIGIMPGHIHKKGRIGIVS RSGTLTYEAVHQTTQVGLGQ SLCVGIGGDPFNGTDFIDCL EIFLNDSATEGIILIGEIGG NAEENAAEFLKQHNSGPNS
Function Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.
Protein Name Succinate--Coa Ligase Adp/Gdp-Forming Subunit Alpha - Mitochondrial
Succinyl-Coa Synthetase Subunit Alpha
Scs-Alpha
Database Links Reactome: R-HSA-71403
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Succinate--Coa Ligase Adp/Gdp-Forming Subunit Alpha - Mitochondrial antibody
Anti-Succinyl-Coa Synthetase Subunit Alpha antibody
Anti-Scs-Alpha antibody
Anti-SUCLG1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance