• Immunoprecipitation analysis of 150 Mu g extracts of HL60 cells using 3 Mu g SUB1 antibody (STJ29150). Western blot was performed from the immunoprecipitate using SUB1 antibody (STJ29150) at a dilution of 1:500.
  • Immunofluorescence analysis of A549 cells using SUB1 Rabbit polyclonal antibody (STJ29150). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.
  • Western blot analysis of various lysates using SUB1 Rabbit polyclonal antibody (STJ29150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SUB1 antibody (1-127) (STJ29150)

SKU:
STJ29150

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUB1 (1-127) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUB1
Gene ID: 10923
Uniprot ID: TCP4_HUMAN
Immunogen Region: 1-127
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human SUB1 (NP_006704.3).
Immunogen Sequence: MPKSKELVSSSSSGSDSDSE VDKKLKRKKQVAPEKPVKKQ KTGETSRALSSSKQSSSSRD DNMFQIGKMRYVSVRDFKGK VLIDIREYWMDPEGEMKPGR KGISLNPEQWSQLKEQISDI DDAVRKL
Post Translational Modifications Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
Function General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
Protein Name Activated Rna Polymerase Ii Transcriptional Coactivator P15
Positive Cofactor 4
Pc4
Sub1 Homolog
P14
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Activated Rna Polymerase Ii Transcriptional Coactivator P15 antibody
Anti-Positive Cofactor 4 antibody
Anti-Pc4 antibody
Anti-Sub1 Homolog antibody
Anti-P14 antibody
Anti-SUB1 antibody
Anti-PC4 antibody
Anti-RPO2TC1 antibody

Information sourced from Uniprot.org

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