| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | STYX |
| Gene ID: | 6815 |
| Uniprot ID: | STYX_HUMAN |
| Immunogen Region: | 1-223 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human STYX (NP_660294.1). |
| Immunogen Sequence: | MEDVKLEFPSLPQCKEDAEE WTYPMRREMQEILPGLFLGP YSSAMKSKLPVLQKHGITHI ICIRQNIEANFIKPNFQQLF RYLVLDIADNPVENIIRFFP MTKEFIDGSLQMGGKVLVHG NAGISRSAAFVIAYIMETFG MKYRDAFAYVQERRFCINPN AGFVHQLQEYEAIYLAKLTI QMMSPLQIERSLSVHSGTTG SLKRTHEEEDDFGTMQVATA QNG |
| Function | Catalytically inactive phosphatase. Acts as a nuclear anchor for MAPK1/MAPK3 (ERK1/ERK2). Modulates cell-fate decisions and cell migration by spatiotemporal regulation of MAPK1/MAPK3 (ERK1/ERK2). By binding to the F-box of FBXW7, prevents the assembly of FBXW7 into the SCF E3 ubiquitin-protein ligase complex, and thereby inhibits degradation of its substrates. Plays a role in spermatogenesis. |
| Protein Name | Serine/Threonine/Tyrosine-Interacting ProteinInactive Tyrosine-Protein Phosphatase StyxPhosphoserine/Threonine/Tyrosine Interaction Protein |
| Cellular Localisation | NucleusCytoplasmCytosolPredominantly Localizes To The Nucleus |
| Alternative Antibody Names | Anti-Serine/Threonine/Tyrosine-Interacting Protein antibodyAnti-Inactive Tyrosine-Protein Phosphatase Styx antibodyAnti-Phosphoserine/Threonine/Tyrosine Interaction Protein antibodyAnti-STYX antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89668/358164/STJ11100730_1__42931.1713122467.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
