• Western blot analysis of extracts of various cell lines, using STX12 antibody (STJ110499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using STX12 antibody (STJ110499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-STX12 antibody (1-276) (STJ110499)

SKU:
STJ110499

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STX12 (1-276) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STX12
Gene ID: 23673
Uniprot ID: STX12_HUMAN
Immunogen Region: 1-276
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-276 of human STX12 (NP_803173.1).
Immunogen Sequence: MSYGPLDMYRNPGPSGPQLR DFSSIIQTCSGNIQRISQAT AQIKNLMSQLGTKQDSSKLQ ENLQQLQHSTNQLAKETNEL LKELGSLPLPLSTSEQRQQR LQKERLMNDFSAALNNFQAV QRRVSEKEKESIARARAGSR LSAEERQREEQLVSFDSHEE WNQMQSQEDEVAITEQDLEL IKERETAIRQLEADILDVNQ IFKDLAMMIHDQGDLIDSIE ANVESSEVHVERATEQLQR
Function SNARE promoting fusion of transport vesicles with target membranes. Together with SNARE STX6, promotes movement of vesicles from endosomes to the cell membrane, and may therefore function in the endocytic recycling pathway. Through complex formation with GRIP1, GRIA2 and NSG1 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting.
Protein Name Syntaxin-12
Cellular Localisation Endosome Membrane
Single-Pass Type Iv Membrane Protein
Golgi Apparatus Membrane
Endomembrane System
Cytoplasmic Side
Early Endosome Membrane
Recycling Endosome Membrane
Alternative Antibody Names Anti-Syntaxin-12 antibody
Anti-STX12 antibody

Information sourced from Uniprot.org

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