• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using STAT4 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from HeLa cells, using STAT4 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Stat4 Polyclonal Antibody

Anti-STAT4 antibody (660-709 aa) (STJ95812)

SKU:
STJ95812

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Signal transducer and activator of transcription 4 (660-709 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IP 2-5 ug mg/lysate
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STAT4
Gene ID: 6775
Uniprot ID: STAT4_HUMAN
Immunogen Region: 660-709 aa
Specificity: Stat4 Polyclonal Antibody detects endogenous levels of Stat4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human STAT4 at the amino acid range 660-709
Function Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response. Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma. Participates also in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps. After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated. Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. Although IL12 appears to be the predominant activating signal, STAT4 can also be phosphorylated and activated in response to IFN-gamma stimulation via JAK1 and TYK2 and in response to different interleukins including IL23, IL2 and IL35. Transcription activation of IFN-gamma gene is mediated by interaction with JUN that forms a complex that efficiently interacts with the AP-1-related sequence of the IFN-gamma promoter. In response to IFN-alpha/beta signaling, acts as a transcriptional repressor and suppresses IL5 and IL13 mRNA expression during response to T-cell receptor (TCR) activation.
Protein Name Signal Transducer And Activator Of Transcription 4
Database Links Reactome: R-HSA-8854691
Reactome: R-HSA-8950505
Reactome: R-HSA-8984722
Reactome: R-HSA-9020591
Reactome: R-HSA-9020933
Reactome: R-HSA-9020958
Cellular Localisation Cytoplasm
Nucleus
Translocated Into The Nucleus In Response To Phosphorylation
Alternative Antibody Names Anti-Signal Transducer And Activator Of Transcription 4 antibody
Anti-STAT4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance