Western blot analysis of lysates from Mouse kidney, using ST8SIA1 Rabbit pAb (STJ116365) at 1:8000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ST8SIA1 antibody (49-270) (STJ116365)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ116365
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General Information

Short DescriptionRabbit polyclonal ST8SIA1 (49-270) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:2000-1:9000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolST8SIA1
Gene ID6489
Uniprot IDSIA8A_HUMAN
Immunogen Region49-270
Immunogen SequenceYRLPNEKEIVQGVLQQGTAW RRNQTAARAFRKQMEDCCDP AHLFAMTKMNSPMGKSMWYD GEFLYSFTIDNSTYSLFPQA TPFQLPLKKCAVVGNGGILK KSGCGRQIDEANFVMRCNLP PLSSEYTKDVGSKSQLVTAN PSIIRQRFQNLLWSRKTFVD NMKIYNHSYIYMPAFSMKTG TEPSLRVYYTLSDVGANQTV LFANPNFLRSIGKFWKSRGI HA
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 49-270 of human ST8SIA1 (NP_003025.1).

Additional Info

Tissue Specificity Strongly expressed in melanoma cell lines, adult and fetal brain and to a lesser extent in adult and fetal lung.
Function Catalyzes the addition of sialic acid in alpha 2,8-linkage to the sialic acid moiety of the ganglioside GM3 to form ganglioside GD3.gangliosides are a subfamily of complex glycosphingolipds that contain one or more residues of sialic acid. Can catalyze the addition of a second alpha-2,8-sialic acid to GD3 to form GT3. Can use GM1b, GD1a and GT1b as acceptor substrates to synthesize GD1c, GT1a and GQ1b respectively. Can synthesize unusual tetra- and pentasialylated lactosylceramide derivatives identified as GQ3 (II3Neu5Ac4-Gg2Cer) and GP3 (II3Neu5Ac5-Gg2Cer) in breast cancer cells.
Protein Name Alpha-N-Acetylneuraminide Alpha-2 -8-Sialyltransferase
Alpha-2 -8-Sialyltransferase 8a
Ganglioside Gd3 Synthase
Ganglioside Gt3 Synthase
Sialyltransferase 8a
Siat8-A
Sialyltransferase St8sia I
St8siai
Database Links Reactome: R-HSA-4085001
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Alpha-N-Acetylneuraminide Alpha-2 -8-Sialyltransferase antibody
Anti-Alpha-2 -8-Sialyltransferase 8a antibody
Anti-Ganglioside Gd3 Synthase antibody
Anti-Ganglioside Gt3 Synthase antibody
Anti-Sialyltransferase 8a antibody
Anti-Siat8-A antibody
Anti-Sialyltransferase St8sia I antibody
Anti-St8siai antibody
Anti-ST8SIA1 antibody
Anti-SIAT8 antibody
Anti-SIAT8A antibody

Information sourced from Uniprot.org

Citations

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