• Immunofluorescence analysis of NIH/3T3 cells using SSR2 Rabbit polyclonal antibody (STJ11103317) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SSR2 Rabbit polyclonal antibody (STJ11103317) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SSR2 in paraffin-embedded rat testis using SSR2 Rabbit polyclonal antibody (STJ11103317) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SSR2 in paraffin-embedded mouse brain using SSR2 Rabbit polyclonal antibody (STJ11103317) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SSR2 Rabbit polyclonal antibody (STJ11103317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SSR2 antibody (18-145) (STJ11103317)

SKU:
STJ11103317

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SSR2 (18-145) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SSR2
Gene ID: 6746
Uniprot ID: SSRB_HUMAN
Immunogen Region: 18-145
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-145 of human SSR2 (NP_003136.1).
Immunogen Sequence: EEGARLLASKSLLNRYAVEG RDLTLQYNIYNVGSSAALDV ELSDDSFPPEDFGIVSGMLN VKWDRIAPASNVSHTVVLRP LKAGYFNFTSATITYLAQED GPVVIGSTSAPGQGGILAQR EFDRRFSP
Function TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.
Protein Name Translocon-Associated Protein Subunit Beta
Trap-Beta
Signal Sequence Receptor Subunit Beta
Ssr-Beta
Database Links Reactome: R-HSA-1799339
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Translocon-Associated Protein Subunit Beta antibody
Anti-Trap-Beta antibody
Anti-Signal Sequence Receptor Subunit Beta antibody
Anti-Ssr-Beta antibody
Anti-SSR2 antibody
Anti-TRAPB antibody
Anti-HSD25 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance