• Immunofluorescence analysis of U-2 OS cells using SRSF7 Rabbit polyclonal antibody (STJ119149) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SRSF7 in paraffin-embedded mouse spinal cord using SRSF7 Rabbit polyclonal antibody (STJ119149) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SRSF7 in paraffin-embedded human esophageal using SRSF7 Rabbit polyclonal antibody (STJ119149) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SRSF7 Rabbit polyclonal antibody (STJ119149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SRSF7 antibody (60-120) (STJ119149)

SKU:
STJ119149

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SRSF7 (60-120) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRSF7
Gene ID: 6432
Uniprot ID: SRSF7_HUMAN
Immunogen Region: 60-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-120 of human SRSF7 (NP_001026854).
Immunogen Sequence: AEDAVRGLDGKVICGSRVRV ELSTGMPRRSRFDRPPARRP FDPNDRCYECGEKGHYAYDC H
Tissue Specificity Brain, liver, kidney and lung.
Post Translational Modifications Extensively phosphorylated on serine residues in the RS domain.
Function Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro. Represses the splicing of MAPT/Tau exon 10. May function as export adapter involved in mRNA nuclear export such as of histone H2A. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway).enhances NXF1-NXT1 RNA-binding activity. RNA-binding is semi-sequence specific.
Protein Name Serine/Arginine-Rich Splicing Factor 7
Splicing Factor 9g8
Splicing Factor - Arginine/Serine-Rich 7
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Serine/Arginine-Rich Splicing Factor 7 antibody
Anti-Splicing Factor 9g8 antibody
Anti-Splicing Factor - Arginine/Serine-Rich 7 antibody
Anti-SRSF7 antibody
Anti-SFRS7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance