Anti-SRGAP2 antibody (Internal) (STJ72107)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-SRGAP2 (Internal) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications. |
| Applications | Pep-ELISA/WB/IF |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 124kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121kDa according to human NP_056141.2 but the rat protein |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | SRGAP2 |
| Gene ID | 23380 |
| Uniprot ID | SRGP2_HUMAN |
| Accession Number | NP_056141.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | KQEDRQTPRSPDST |
| Specificity | This antibody is expected to recognize isoform a (NP_056141.2). |
Additional Info
| Function | Postsynaptic RAC1 GTPase activating protein (GAP) that plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex. Regulates excitatory and inhibitory synapse maturation and density in cortical pyramidal neurons. SRGAP2/SRGAP2A limits excitatory and inhibitory synapse density through its RAC1-specific GTPase activating activity, while it promotes maturation of both excitatory and inhibitory synapses through its ability to bind to the postsynaptic scaffolding protein HOMER1 at excitatory synapses, and the postsynaptic protein GPHN at inhibitory synapses. Mechanistically, acts by binding and deforming membranes, thereby regulating actin dynamics to regulate cell migration and differentiation. Promotes cell repulsion and contact inhibition of locomotion: localizes to protrusions with curved edges and controls the duration of RAC1 activity in contact protrusions. In non-neuronal cells, may also play a role in cell migration by regulating the formation of lamellipodia and filopodia. |
| Protein Name | Slit-Robo Rho Gtpase-Activating Protein 2Srgap2Formin-Binding Protein 2Rho Gtpase-Activating Protein 34 |
| Database Links | Reactome: R-HSA-428543Reactome: R-HSA-5663220Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013406Reactome: R-HSA-9013420Reactome: R-HSA-9013423Reactome: R-HSA-9035034 |
| Cellular Localisation | Cell MembraneCell ProjectionDendritic SpinePostsynaptic DensityPostsynaptic Cell MembraneLamellipodiumCytoplasmic VesiclePhagosomeNucleusCytoplasmCytosolRecruited To Actin-Rich Phagosomes During PhagocytosisTranslocates From Nucleus To Cytoplasm During Development |
| Alternative Antibody Names | Anti-Slit-Robo Rho Gtpase-Activating Protein 2 antibodyAnti-Srgap2 antibodyAnti-Formin-Binding Protein 2 antibodyAnti-Rho Gtpase-Activating Protein 34 antibodyAnti-SRGAP2 antibodyAnti-ARHGAP34 antibodyAnti-FNBP2 antibodyAnti-KIAA0456 antibodyAnti-SRGAP2A antibody |
Information sourced from Uniprot.org