• Immunofluorescence analysis of paraffin-embedded rat testis using SRD5A2 Rabbit monoclonal antibody (STJ11103118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded mouse testis using SRD5A2 Rabbit monoclonal antibody (STJ11103118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SRD5A2 Rabbit monoclonal antibody (STJ11103118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SRD5A2 antibody (1-100) [S8MR] (STJ11103118)

SKU:
STJ11103118

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SRD5A2 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRD5A2
Gene ID: 6716
Uniprot ID: S5A2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRD5A2 (P31213).
Immunogen Sequence: MQVQCQQSPVLAGSATLVAL GALALYVAKPSGYGKHTESL KPAATRLPARAAWFLQELPS FAVPAGILARQPLSLFGPPG TVLLGLFCVHYFHRTFVYSL
Tissue Specificity Expressed in high levels in the prostate and many other androgen-sensitive tissues.
Function Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Protein Name 3-Oxo-5-Alpha-Steroid 4-Dehydrogenase 2
5 Alpha-Sr2
Sr Type 2
Steroid 5-Alpha-Reductase 2
S5ar 2
Type Ii 5-Alpha Reductase
Database Links Reactome: R-HSA-193048
Cellular Localisation Microsome Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-3-Oxo-5-Alpha-Steroid 4-Dehydrogenase 2 antibody
Anti-5 Alpha-Sr2 antibody
Anti-Sr Type 2 antibody
Anti-Steroid 5-Alpha-Reductase 2 antibody
Anti-S5ar 2 antibody
Anti-Type Ii 5-Alpha Reductase antibody
Anti-SRD5A2 antibody

Information sourced from Uniprot.org

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