• Western blot analysis of lysates from DU145 cells, using SPZ1 Rabbit polyclonal antibody (STJ11102929) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SPZ1 antibody (1-430) (STJ11102929)

SKU:
STJ11102929

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPZ1 (1-430) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPZ1
Gene ID: 84654
Uniprot ID: SPZ1_HUMAN
Immunogen Region: 1-430
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-430 of human SPZ1 (NP_115956.3).
Immunogen Sequence: MASSAKSAEMPTISKTVNPT PDPHQEYLDPRITIALFEIG SHSPSSWGSLPFLKNSSHQV TEQQTAQKFNNLLKEIKDIL KNMAGFEEKITEAKELFEET NITEDVSAHKENIRGLDKIN EMLSTNLPVSLAPEKEDNEK KQEMILETNITEDVSAHKEN IRGLDKINEMLSTNLPVSLA PEKEDNEKKQQMIMENQNSE NTAQVFARDLVNRLEEKKVL NETQQSQEKAKNRLNVQEE
Tissue Specificity Specifically and strongly expressed in the testis. Expressed in several tumor cell lines.
Post Translational Modifications Phosphorylated by MAPK1/ERK2 and MAPK3/ERK1.
Function Transcription factor that binds to the DNA sequence 5'-CANNTG-3'(E box) and the G-box motif. May play an important role in the regulation of cell proliferation and differentiation during spermatogenesis.
Protein Name Spermatogenic Leucine Zipper Protein 1
Testis-Specific Protein 1
Testis-Specific Protein Nyd-Tsp1
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Spermatogenic Leucine Zipper Protein 1 antibody
Anti-Testis-Specific Protein 1 antibody
Anti-Testis-Specific Protein Nyd-Tsp1 antibody
Anti-SPZ1 antibody
Anti-TSP1 antibody

Information sourced from Uniprot.org

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