• Immunofluorescence analysis of paraffin-embedded mouse skin using SPRR1A Rabbit polyclonal antibody (STJ119627) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of Mouse stomach, using SPRR1A Rabbit polyclonal antibody (STJ119627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SPRR1A antibody (73-89) (STJ119627)

SKU:
STJ119627

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPRR1A (73-89) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPRR1A
Gene ID: 6698
Uniprot ID: SPR1A_HUMAN
Immunogen Region: 73-89
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 73-89 of human SPRR1A (NP_005978.2).
Immunogen Sequence: CPSTVTPAPAQQKTKQK
Function Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane.
Protein Name Cornifin-A
19 Kda Pancornulin
Sprk
Small Proline-Rich Protein Ia
Spr-Ia
Database Links Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Cornifin-A antibody
Anti-19 Kda Pancornulin antibody
Anti-Sprk antibody
Anti-Small Proline-Rich Protein Ia antibody
Anti-Spr-Ia antibody
Anti-SPRR1A antibody

Information sourced from Uniprot.org

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