Host: | Goat |
Applications: | Pep-ELISA/WB/IF/FC/IHC |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Goat polyclonal antibody anti-Sprouty (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 37kDa band observed in Human Kidney lysates (calculated MW of 35.1kDa according to NP_005832.1 ). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temper |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | SPRY1 |
Gene ID: | 10252 |
Uniprot ID: | SPY1_HUMAN |
Immunogen Region: | C-Term |
Accession Number: | NP_005832.1; NP_955359.1 |
Specificity: | NP_005832.1 and NP_955359.1 represent varients of the same protein. |
Immunogen Sequence: | CPSRGQGKPS |
Protein Name | Protein Sprouty Homolog 1Spry-1 |
Database Links | Reactome: R-HSA-182971 |
Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinFound In The Cytoplasm In Unstimulated Cells But Is Translocated To The Membrane Ruffles In Cells Stimulated With Egf (Epidermal Growth Factor) |
Alternative Antibody Names | Anti-Protein Sprouty Homolog 1 antibodyAnti-Spry-1 antibodyAnti-SPRY1 antibody |
Information sourced from Uniprot.org