Anti-SPPL2A antibody (421-520) (STJ117734)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SPPL2A (421-520) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SPPL2A |
| Gene ID | 84888 |
| Uniprot ID | SPP2A_HUMAN |
| Immunogen Region | 421-520 |
| Immunogen Sequence | AYCRRFDVQTGSSYIYYVSS TVAYAIGMILTFVVLVLMKK GQPALLYLVPCTLITASVVA WRRKEMKKFWKGNSYQMMDH LDCATNEENPVISGEQIVQQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 421-520 of human SPPL2A (NP_116191.2). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Glycosylated. |
| Function | Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). Essential for degradation of the invariant chain CD74 that plays a central role in the function of antigen-presenting cells in the immune system. Plays a role in the regulation of innate and adaptive immunity. Catalyzes the intramembrane cleavage of the simian foamy virus envelope glycoprotein gp130 independently of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis. |
| Protein Name | Signal Peptide Peptidase-Like 2aSpp-Like 2aSppl2aIntramembrane Protease 3Imp-3Presenilin-Like Protein 2 |
| Database Links | Reactome: R-HSA-5357905 |
| Cellular Localisation | Late Endosome MembraneMulti-Pass Membrane ProteinLysosome MembraneMembraneLumenal SideColocalizes With Palmitoylated And Myristoylated Proteins At The Plasma Membrane |
| Alternative Antibody Names | Anti-Signal Peptide Peptidase-Like 2a antibodyAnti-Spp-Like 2a antibodyAnti-Sppl2a antibodyAnti-Intramembrane Protease 3 antibodyAnti-Imp-3 antibodyAnti-Presenilin-Like Protein 2 antibodyAnti-SPPL2A antibodyAnti-IMP3 antibodyAnti-PSL2 antibodyAnti-PSEC0147 antibody |
Information sourced from Uniprot.org