| Short Description : | Rabbit polyclonal SPIRE2 (1-190) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SPIRE2 |
| Gene ID: | 84501 |
| Uniprot ID: | SPIR2_HUMAN |
| Immunogen Region: | 1-190 |
| Immunogen Sequence: | MARAGSCGGAAAGAGRPEPW ELSLEEVLKAYEQPLNEEQA WAVCFQGCRGLRGSPGRRLR DTGDLLLRGDGSVGAREPEA AEPATMVVPLASSEAQTVQS LGFAIYRALDWGLDESEERE LSPQLERLIDLMANNDSEDS GCGAADEGYGGPEEEEEAEG VPRSVRTFAQAMRLCAARLT DPRGAQAHYQ |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human SPIRE2 (NP_115827.1). |
| Function | Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis. Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage. |
| Protein Name | Protein Spire Homolog 2Spir-2 |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonCytosolCell MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmic Vesicle MembraneDetected At The Cleavage Furrow During Asymmetric Oocyte Division And Polar Body Extrusion |
| Alternative Antibody Names | Anti-Protein Spire Homolog 2 antibodyAnti-Spir-2 antibodyAnti-SPIRE2 antibodyAnti-KIAA1832 antibodyAnti-SPIR2 antibody |
Information sourced from Uniprot.org
