| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SPIRE2 (1-190) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SPIRE2 |
| Gene ID: |
84501 |
| Uniprot ID: |
SPIR2_HUMAN |
| Immunogen Region: |
1-190 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human SPIRE2 (NP_115827.1). |
| Immunogen Sequence: |
MARAGSCGGAAAGAGRPEPW ELSLEEVLKAYEQPLNEEQA WAVCFQGCRGLRGSPGRRLR DTGDLLLRGDGSVGAREPEA AEPATMVVPLASSEAQTVQS LGFAIYRALDWGLDESEERE LSPQLERLIDLMANNDSEDS GCGAADEGYGGPEEEEEAEG VPRSVRTFAQAMRLCAARLT DPRGAQAHYQ |
| Function | Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis. Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage. |
| Protein Name | Protein Spire Homolog 2Spir-2 |
| Cellular Localisation | CytoplasmCytoskeletonCytosolCell MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmic Vesicle MembraneDetected At The Cleavage Furrow During Asymmetric Oocyte Division And Polar Body Extrusion |
| Alternative Antibody Names | Anti-Protein Spire Homolog 2 antibodyAnti-Spir-2 antibodyAnti-SPIRE2 antibodyAnti-KIAA1832 antibodyAnti-SPIR2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/96581/379140/STJ117622_1__97810.1713146869.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")