Anti-Sphingosine Kinase 1 antibody (C-Term) [M540] (STJA0041387)
SPECIFICATIONS
ClonalityMonoclonal
HostRat
ConjugationUnconjugated
IsotypeN.T.
ImmunogenClone M540 was generated from a SK1 synthetic peptide (coupled to carrier) corresponding to amino acids in the C-terminal region of mouse SK1. This sequence has high homology to human and rat SK1, and is not homologous to sequences in SK2.
General Information
| Short Description | Rat monoclonal anti-Sphingosine Kinase 1 (C-Term) for use in ELISA, WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC |
| Host | Rat |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M540 |
| Isotype | N.T. |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:250ICC 1:200 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20ยฐC is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20ยฐC. |
Target Information
| Gene Symbol | Sphk1 |
| Gene ID | 20698 |
| Uniprot ID | SPHK1_MOUSE |
| Immunogen | Clone M540 was generated from a SK1 synthetic peptide (coupled to carrier) corresponding to amino acids in the C-terminal region of mouse SK1. This sequence has high homology to human and rat SK1, and is not homologous to sequences in SK2. |
| Immunogen Region | C-Term |
| Immunogen Sequence | Mouse |
| Specificity | The antibody detects 42 kDa* full-length recombinant human and mouse SK1 proteins, as well as detects 42, 50, and 60 kDa forms of SK1 in human HeLa cells. |
Additional Info
| Tissue Specificity | Widely expressed. Expressed in brain (at protein level). Detected in neurons. |
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA. Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. |
| Protein Name | Sphingosine Kinase 1Sk 1Spk 1Acetyltransferase Sphk1 |
| Database Links | Reactome: R-MMU-1660661Reactome: -MMU-390471Reactome: -MMU-5218921Reactome: -MMU-9009391Reactome: -MMU-9833482 |
| Cellular Localisation | CytoplasmEndosome MembranePeripheral Membrane ProteinNucleusCell MembraneSynapseTranslocated From The Cytoplasm To The Plasma Membrane In A Cib1-Dependent MannerBinds To Membranes Containing Negatively Charged Lipids But Not Neutral LipidsRecruited To Endocytic Membranes By Sphingosine Where Promotes Membrane Fusion |
| Alternative Antibody Names | Anti-Sphingosine Kinase 1 antibodyAnti-Sk 1 antibodyAnti-Spk 1 antibodyAnti-Acetyltransferase Sphk1 antibodyAnti-Sphk1 antibodyAnti-Sk1 antibody |
Information sourced from Uniprot.org